A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen Lasiodiplodia theobromae.
Identifieur interne : 000107 ( Main/Exploration ); précédent : 000106; suivant : 000108A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen Lasiodiplodia theobromae.
Auteurs : Dulanjalee Lakmali Harishchandra [République populaire de Chine, Thaïlande] ; Wei Zhang [République populaire de Chine] ; Xinghong Li [République populaire de Chine] ; Kandawatte Wedaralalage Thilini Chethana [Thaïlande] ; Kevin David Hyde [Thaïlande] ; Siraprapa Brooks [Thaïlande] ; Jiye Yan [République populaire de Chine] ; Junbo Peng [République populaire de Chine]Source :
- The plant pathology journal [ 1598-2254 ] ; 2020.
Abstract
Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted N-glycosylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of LtLysM1 displayed increased virulence on grapevine shoots in comparison with that of wild type CSS-01s, and RNAi transformants of LtLysM1 exhibited significantly decreased lesion length when compared with that of wild type CSS-01s. Moreover, LtLysM1 was confirmed to be a secreted protein by a yeast signal peptide trap assay. Transient expression in Nicotiana benthamiana together with protein immunoblotting confirmed that LtLysM1 was an N-glycosylated protein. In contrast to previously reported LysM protein Slp1 and OsCEBiP, LtLysM1 molecule did not interact with itself based on yeast two hybrid and co-immunoprecipitation assays. These results indicate that LtLysM1 is a secreted protein and functions as a critical virulence factor during the disease symptom development in woody plants.
DOI: 10.5423/PPJ.OA.05.2020.0084
PubMed: 32788891
PubMed Central: PMC7403516
Affiliations:
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China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation><affiliation wicri:level="1"><nlm:affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100</wicri:regionArea><wicri:noRegion>Chiang Rai 57100</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Li, Xinghong" sort="Li, Xinghong" uniqKey="Li X" first="Xinghong" last="Li">Xinghong Li</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Chethana, Kandawatte Wedaralalage Thilini" sort="Chethana, Kandawatte Wedaralalage Thilini" uniqKey="Chethana K" first="Kandawatte Wedaralalage Thilini" last="Chethana">Kandawatte Wedaralalage Thilini Chethana</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100</wicri:regionArea><wicri:noRegion>Chiang Rai 57100</wicri:noRegion></affiliation></author><author><name sortKey="Hyde, Kevin David" sort="Hyde, Kevin David" uniqKey="Hyde K" first="Kevin David" last="Hyde">Kevin David Hyde</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100</wicri:regionArea><wicri:noRegion>Chiang Rai 57100</wicri:noRegion></affiliation></author><author><name sortKey="Brooks, Siraprapa" sort="Brooks, Siraprapa" uniqKey="Brooks S" first="Siraprapa" last="Brooks">Siraprapa Brooks</name><affiliation wicri:level="1"><nlm:affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</nlm:affiliation><country xml:lang="fr">Thaïlande</country><wicri:regionArea>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100</wicri:regionArea><wicri:noRegion>Chiang Rai 57100</wicri:noRegion></affiliation></author><author><name sortKey="Yan, Jiye" sort="Yan, Jiye" uniqKey="Yan J" first="Jiye" last="Yan">Jiye Yan</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Peng, Junbo" sort="Peng, Junbo" uniqKey="Peng J" first="Junbo" last="Peng">Junbo Peng</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author></analytic><series><title level="j">The plant pathology journal</title><idno type="ISSN">1598-2254</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen <i>Lasiodiplodia theobromae.</i> Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted <i>N</i>-glycosylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of <i>LtLysM1</i> displayed increased virulence on grapevine shoots in comparison with that of wild type CSS-01s, and RNAi transformants of <i>LtLysM1</i> exhibited significantly decreased lesion length when compared with that of wild type CSS-01s. Moreover, LtLysM1 was confirmed to be a secreted protein by a yeast signal peptide trap assay. Transient expression in <i>Nicotiana benthamiana</i> together with protein immunoblotting confirmed that LtLysM1 was an <i>N</i>-glycosylated protein. In contrast to previously reported LysM protein Slp1 and OsCEBiP, LtLysM1 molecule did not interact with itself based on yeast two hybrid and co-immunoprecipitation assays. These results indicate that LtLysM1 is a secreted protein and functions as a critical virulence factor during the disease symptom development in woody plants.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">32788891</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>28</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1598-2254</ISSN><JournalIssue CitedMedium="Print"><Volume>36</Volume><Issue>4</Issue><PubDate><Year>2020</Year><Month>Aug</Month><Day>01</Day></PubDate></JournalIssue><Title>The plant pathology journal</Title><ISOAbbreviation>Plant Pathol J</ISOAbbreviation></Journal><ArticleTitle>A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen <i>Lasiodiplodia theobromae</i>.</ArticleTitle><Pagination><MedlinePgn>323-334</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.5423/PPJ.OA.05.2020.0084</ELocationID><Abstract><AbstractText>Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen <i>Lasiodiplodia theobromae.</i> Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence analyses revealed that LtLysM1 was a putative glycoprotein with 10 predicted <i>N</i>-glycosylation sites and one LysM domain. Pathogenicity tests showed that overexpressed transformants of <i>LtLysM1</i> displayed increased virulence on grapevine shoots in comparison with that of wild type CSS-01s, and RNAi transformants of <i>LtLysM1</i> exhibited significantly decreased lesion length when compared with that of wild type CSS-01s. Moreover, LtLysM1 was confirmed to be a secreted protein by a yeast signal peptide trap assay. Transient expression in <i>Nicotiana benthamiana</i> together with protein immunoblotting confirmed that LtLysM1 was an <i>N</i>-glycosylated protein. In contrast to previously reported LysM protein Slp1 and OsCEBiP, LtLysM1 molecule did not interact with itself based on yeast two hybrid and co-immunoprecipitation assays. These results indicate that LtLysM1 is a secreted protein and functions as a critical virulence factor during the disease symptom development in woody plants.</AbstractText><CopyrightInformation>© The Korean Society of Plant Pathology.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Harishchandra</LastName><ForeName>Dulanjalee Lakmali</ForeName><Initials>DL</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Wei</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xinghong</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chethana</LastName><ForeName>Kandawatte Wedaralalage Thilini</ForeName><Initials>KWT</Initials><AffiliationInfo><Affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hyde</LastName><ForeName>Kevin David</ForeName><Initials>KD</Initials><AffiliationInfo><Affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brooks</LastName><ForeName>Siraprapa</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Centre of Excellence in Fungal Research, School of Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yan</LastName><ForeName>Jiye</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Peng</LastName><ForeName>Junbo</ForeName><Initials>J</Initials><Identifier Source="ORCID">https://orcid.org/0000-0002-1872-0968</Identifier><AffiliationInfo><Affiliation>Beijing Key Laboratory of Environment Friendly Management on Fruit Diseases and Pests in North China, Institute of Plant and Environment Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Korea (South)</Country><MedlineTA>Plant Pathol J</MedlineTA><NlmUniqueID>100966769</NlmUniqueID><ISSNLinking>1598-2254</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">N-glycosylation</Keyword><Keyword MajorTopicYN="N">grapevine</Keyword><Keyword 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IdType="doi">10.5423/PPJ.OA.05.2020.0084</ArticleId><ArticleId IdType="pii">PPJ-36-323</ArticleId><ArticleId IdType="pmc">PMC7403516</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>Nucleic Acids Res. 2019 Jan 8;47(D1):D427-D432</Citation><ArticleIdList><ArticleId IdType="pubmed">30357350</ArticleId></ArticleIdList></Reference><Reference><Citation>Elife. 2013 Jul 02;2:e00790</Citation><ArticleIdList><ArticleId IdType="pubmed">23840930</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Microbiol. 2008 May;68(4):838-47</Citation><ArticleIdList><ArticleId IdType="pubmed">18430080</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2020 Jan;225(1):448-460</Citation><ArticleIdList><ArticleId IdType="pubmed">31596956</ArticleId></ArticleIdList></Reference><Reference><Citation>Microbes Infect. 2011 Dec;13(14-15):1168-76</Citation><ArticleIdList><ArticleId IdType="pubmed">21856436</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2019 Sep 25;10:2231</Citation><ArticleIdList><ArticleId IdType="pubmed">31608044</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2019 Jun 26;10:822</Citation><ArticleIdList><ArticleId IdType="pubmed">31297126</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS One. 2011;6(11):e27217</Citation><ArticleIdList><ArticleId IdType="pubmed">22076138</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2016 Sep;211(4):1323-37</Citation><ArticleIdList><ArticleId IdType="pubmed">27174033</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Microbe Interact. 2016 May;29(5):405-16</Citation><ArticleIdList><ArticleId IdType="pubmed">26927000</ArticleId></ArticleIdList></Reference><Reference><Citation>New Phytol. 2016 Dec;212(4):896-901</Citation><ArticleIdList><ArticleId IdType="pubmed">27329426</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2013 May 16;4:139</Citation><ArticleIdList><ArticleId IdType="pubmed">23720666</ArticleId></ArticleIdList></Reference><Reference><Citation>Annu Rev Phytopathol. 2009;47:233-63</Citation><ArticleIdList><ArticleId IdType="pubmed">19400631</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Microbiol. 2016 Apr 27;7:600</Citation><ArticleIdList><ArticleId IdType="pubmed">27199930</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2010 Aug 20;329(5994):953-5</Citation><ArticleIdList><ArticleId IdType="pubmed">20724636</ArticleId></ArticleIdList></Reference><Reference><Citation>PLoS Pathog. 2013;9(12):e1003769</Citation><ArticleIdList><ArticleId IdType="pubmed">24348247</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2019 Sep 11;9(1):13144</Citation><ArticleIdList><ArticleId IdType="pubmed">31511626</ArticleId></ArticleIdList></Reference><Reference><Citation>Trends Immunol. 2014 Jul;35(7):345-51</Citation><ArticleIdList><ArticleId IdType="pubmed">24946686</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2011 Jan;23(1):4-15</Citation><ArticleIdList><ArticleId IdType="pubmed">21278123</ArticleId></ArticleIdList></Reference><Reference><Citation>DNA Res. 2018 Feb 1;25(1):87-102</Citation><ArticleIdList><ArticleId IdType="pubmed">29036669</ArticleId></ArticleIdList></Reference><Reference><Citation>Gene. 1997 Oct 1;198(1-2):289-96</Citation><ArticleIdList><ArticleId IdType="pubmed">9370294</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2020 Jan 21;10(1):865</Citation><ArticleIdList><ArticleId IdType="pubmed">31964981</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Genet. 2015 May;61(2):103-13</Citation><ArticleIdList><ArticleId IdType="pubmed">25589417</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2012 Jun 1;336(6085):1160-4</Citation><ArticleIdList><ArticleId IdType="pubmed">22654057</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Physiol. 2011 Jun;156(2):756-69</Citation><ArticleIdList><ArticleId IdType="pubmed">21467214</ArticleId></ArticleIdList></Reference><Reference><Citation>Int J Mol Sci. 2019 Dec 03;20(23):</Citation><ArticleIdList><ArticleId IdType="pubmed">31816814</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 1996 Jul 9;93(14):7108-13</Citation><ArticleIdList><ArticleId IdType="pubmed">8692953</ArticleId></ArticleIdList></Reference><Reference><Citation>Sci Rep. 2016 May 31;6:26951</Citation><ArticleIdList><ArticleId IdType="pubmed">27243217</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Cell. 2014 Apr 24;54(2):263-72</Citation><ArticleIdList><ArticleId IdType="pubmed">24766890</ArticleId></ArticleIdList></Reference><Reference><Citation>Cold Spring Harb Symp Quant Biol. 2012;77:235-47</Citation><ArticleIdList><ArticleId IdType="pubmed">23223409</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Dis. 2008 Apr;92(4):519-529</Citation><ArticleIdList><ArticleId IdType="pubmed">30769642</ArticleId></ArticleIdList></Reference><Reference><Citation>Front Plant Sci. 2019 Nov 14;10:1437</Citation><ArticleIdList><ArticleId IdType="pubmed">31803201</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2012 Jan;24(1):322-35</Citation><ArticleIdList><ArticleId IdType="pubmed">22267486</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2009 Sep;21(9):2928-47</Citation><ArticleIdList><ArticleId IdType="pubmed">19794118</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods Mol Biol. 2012;835:519-30</Citation><ArticleIdList><ArticleId IdType="pubmed">22183675</ArticleId></ArticleIdList></Reference><Reference><Citation>BMC Genomics. 2016 Aug 11;17(1):615</Citation><ArticleIdList><ArticleId IdType="pubmed">27514986</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant J. 2010 Oct;64(2):204-14</Citation><ArticleIdList><ArticleId IdType="pubmed">21070404</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Cell. 2014 Mar;26(3):1360-76</Citation><ArticleIdList><ArticleId IdType="pubmed">24642938</ArticleId></ArticleIdList></Reference><Reference><Citation>Mol Plant Pathol. 2017 May;18(4):596-608</Citation><ArticleIdList><ArticleId IdType="pubmed">27911046</ArticleId></ArticleIdList></Reference><Reference><Citation>Methods. 2001 Dec;25(4):402-8</Citation><ArticleIdList><ArticleId IdType="pubmed">11846609</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2006 Nov 16;444(7117):323-9</Citation><ArticleIdList><ArticleId IdType="pubmed">17108957</ArticleId></ArticleIdList></Reference><Reference><Citation>Plant Signal Behav. 2019;14(2):1557008</Citation><ArticleIdList><ArticleId IdType="pubmed">30621489</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>Thaïlande</li></country><settlement><li>Pékin</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Harishchandra, Dulanjalee Lakmali" sort="Harishchandra, Dulanjalee Lakmali" uniqKey="Harishchandra D" first="Dulanjalee Lakmali" last="Harishchandra">Dulanjalee Lakmali Harishchandra</name></noRegion><name sortKey="Li, Xinghong" sort="Li, Xinghong" uniqKey="Li X" first="Xinghong" last="Li">Xinghong Li</name><name sortKey="Peng, Junbo" sort="Peng, Junbo" uniqKey="Peng J" first="Junbo" last="Peng">Junbo Peng</name><name sortKey="Yan, Jiye" sort="Yan, Jiye" uniqKey="Yan J" first="Jiye" last="Yan">Jiye Yan</name><name sortKey="Zhang, Wei" sort="Zhang, Wei" uniqKey="Zhang W" first="Wei" last="Zhang">Wei Zhang</name></country><country name="Thaïlande"><noRegion><name sortKey="Harishchandra, Dulanjalee Lakmali" sort="Harishchandra, Dulanjalee Lakmali" uniqKey="Harishchandra D" first="Dulanjalee Lakmali" last="Harishchandra">Dulanjalee Lakmali Harishchandra</name></noRegion><name sortKey="Brooks, Siraprapa" sort="Brooks, Siraprapa" uniqKey="Brooks S" first="Siraprapa" last="Brooks">Siraprapa Brooks</name><name sortKey="Chethana, Kandawatte Wedaralalage Thilini" sort="Chethana, Kandawatte Wedaralalage Thilini" uniqKey="Chethana K" first="Kandawatte Wedaralalage Thilini" last="Chethana">Kandawatte Wedaralalage Thilini Chethana</name><name sortKey="Hyde, Kevin David" sort="Hyde, Kevin David" uniqKey="Hyde K" first="Kevin David" last="Hyde">Kevin David Hyde</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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